SKU:SEC920Hu
ELISA Kit for Platelet Derived Growth Factor C (PDGFC)
ELISA Kit for Platelet Derived Growth Factor C (PDGFC)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEC920Hu
Organism Species
Homo sapiens (Human).
Sample Type
Plasma.
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
125.00-8000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 43pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor C (PDGFC).
No significant cross-reactivity or interference between Platelet Derived Growth Factor C (PDGFC) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Platelet Derived Growth Factor C (PDGFC) and the recovery rates were calculated by comparing the measured value to the expected amount of Platelet Derived Growth Factor C (PDGFC) in samples.
Matrix | Recovery range (%) | Average(%) |
sodium citrate plasma(n=5) | 85-101 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor C (PDGFC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor C (PDGFC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Platelet Derived Growth Factor C (PDGFC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
sodium citrate plasma(n=5) | 88-96% | 80-97% | 97-105% | 80-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Magazine | Citations |
Immunobiology | Platelet derived growth factor-CC secreted by M2 macrophages induces alpha-smooth muscle actin expression by dermal and gingival fibroblasts PubMed: 23182716 |
Physiological Reports | High glucose induces plateletâderived growth factorâC via carbohydrate response elementâbinding protein in glomerular mesangial cells Pubmed:27033449 |
Respiratory Research | Changes in pulmonary endothelial cell properties during bleomycin-induced pulmonary fibrosis Pubmed:29940932 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPC920Hu01 | Recombinant Platelet Derived Growth Factor C (PDGFC) | Positive Control; Immunogen; SDS-PAGE; WB. |
APC920Hu01 | Active Platelet Derived Growth Factor C (PDGFC) | Cell culture; Activity Assays. |
PAC920Hu01 | Polyclonal Antibody to Platelet Derived Growth Factor C (PDGFC) | WB; IHC; ICC; IP. |
MAC920Hu22 | Monoclonal Antibody to Platelet Derived Growth Factor C (PDGFC) | WB; IHC; ICC; IP. |
SEC920Hu | ELISA Kit for Platelet Derived Growth Factor C (PDGFC) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCC920Hu | CLIA Kit for Platelet Derived Growth Factor C (PDGFC) | Chemiluminescent immunoassay for Antigen Detection. |
LMC920Hu | Magnetic Luminex Assay Kit for Platelet Derived Growth Factor C (PDGFC) ,etc. | Magnetic Luminex Assay for Antigen Detection. |