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CLIA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM)
CLIA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM)
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Chemiluminescent immunoassay for Antigen Detection.
Product No.
SCA002Hu
Organism Species
Homo sapiens (Human).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
2h, 40min
Detection Range
27.4-20,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 12.9pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Activated Leukocyte Cell Adhesion Molecule (ALCAM).
No significant cross-reactivity or interference between Activated Leukocyte Cell Adhesion Molecule (ALCAM) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Activated Leukocyte Cell Adhesion Molecule (ALCAM) and the recovery rates were calculated by comparing the measured value to the expected amount of Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-105 | 102 |
| EDTA plasma(n=5) | 83-97 | 87 |
| heparin plasma(n=5) | 84-96 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Activated Leukocyte Cell Adhesion Molecule (ALCAM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-101% | 95-103% | 93-101% | 94-105% |
| EDTA plasma(n=5) | 82-99% | 83-101% | 79-91% | 80-95% |
| heparin plasma(n=5) | 99-105% | 81-90% | 83-99% | 83-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Substrate A | 1×10mL | Substrate B | 1×2mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
| Magazine | Citations |
| Breast | Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator PubMed: 26446295 |
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