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SKU:SEB973Ra
ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)
ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEB973Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
78.12-5000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 30.5pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1).
No significant cross-reactivity or interference between A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) and the recovery rates were calculated by comparing the measured value to the expected amount of A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 81-104 | 87 |
| heparin plasma(n=5) | 91-98 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-104% | 83-92% | 80-94% | 82-104% |
| EDTA plasma(n=5) | 92-104% | 84-95% | 86-93% | 94-102% |
| heparin plasma(n=5) | 91-105% | 83-93% | 94-101% | 83-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| PLos One | Breast Cancer Cells Induce Stromal Fibroblasts to Secrete ADAMTS1 for Cancer Invasion through an Epigenetic Change Plosone: 0035128 |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748 |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748 |
| JOURNAL OF PERIODONTOLOGY | A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A … Pubmed: 31020669 |
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