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SKU:SCA097Ra
CLIA Kit for Matrix Metalloproteinase 1 (MMP1)
CLIA Kit for Matrix Metalloproteinase 1 (MMP1)
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Chemiluminescent immunoassay for Antigen Detection.
Product No.
SCA097Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
2h, 40min
Detection Range
13.7-10,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 4.9pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 1 (MMP1).
No significant cross-reactivity or interference between Matrix Metalloproteinase 1 (MMP1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 1 (MMP1) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 1 (MMP1) in samples.
|
Matrix |
Recovery range (%) |
Average(%) |
|
serum(n=5) |
91-101 |
96 |
|
heparin plasma(n=5) |
91-99 |
96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 1 (MMP1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
88-103% |
85-92% |
78-92% |
89-96% |
|
heparin plasma(n=5) |
80-90% |
84-99% |
80-98% |
82-92% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|
Reagents |
Quantity |
Reagents |
Quantity |
|
Pre-coated, ready to use 96-well strip plate |
1 |
Plate sealer for 96 wells |
4 |
|
Standard |
2 |
Standard Diluent |
1×20mL |
|
Detection Reagent A |
1×120µL |
Assay Diluent A |
1×12mL |
|
Detection Reagent B |
1×120µL |
Assay Diluent B |
1×12mL |
|
Substrate A |
1×10mL |
Substrate B |
1×2mL |
|
Wash Buffer (30 × concentrate) |
1×20mL |
Instruction manual |
1 |
Assay procedure summary
-
Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
|
Magazine |
Citations |
|
Parasitology Research |
Therapeutic effect of alpha lipoic acid combined with praziquantel on liver fibrosis induced by Schistosoma mansoni challenged mice SpringerLink: j33n80517577535k |
|
Infection and Immunity |
Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier Pubmed: 23649093 |
|
PLoS ONE |
A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
|
Thromb Haemost. |
Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655 |
|
The Journal of Pathology |
Mycobacterium tuberculosis dysregulates MMP/TIMP balance to drive rapid cavitation and unrestrained bacterial proliferation Pubmed:25186281 |
|
Connective Tissue Research |
Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747 |
|
J Immunol |
Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis PubMed: 26268658 |
|
Cellular & Molecular Immunology |
Effect of ebosin on modulating interleukin-1&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes PubMed: 25938977 |
|
J Periodontal Implant Sci |
Assessment of MMP-1, MMP-8 and TIMP-2 in experimental periodontitis treated with kaempferol pmc:PMC4848383 |
|
Int Wound J. |
From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748 |
|
Cellular & Molecular Immunology |
Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes pubmed:25938977 |
|
Molecular Medicine Reports |
Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells pubmed:28447732 |
|
Analytical Methods |
The development of a matrix metalloproteinase-1 biosensor based on the surface plasmon resonance imaging technique c6ay01856d |
|
Journal of Cellular Physiology |
Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models pubmed:27739057 |
|
Journal of Applied Animal Research |
Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis 10.108:09712119.2017.1335208 |
|
Analytical Methods |
SPRI biosensors for quantitative determination of matrix metalloproteinase-2 DOI:10.1039/C7AY00786H |
|
International Wound Journal |
From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748 |
|
European Heart Journal |
Biomarker‐based phenotyping of myocardial fibrosis identifies patients with heart failure with preserved ejection fraction resistant to the beneficial effects of … Pubmed:29709099 |
|
Journal of Agricultural and Food Chemistry |
Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type I procollagen production in mice Pubmed:30032605 |
|
Oral Diseases |
TIMP‐1 association with collagen type I overproduction in hereditary gingival fibromatosis Pubmed:29989318 |
|
Journal of Applied Animal Research |
Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis 10.1080:09712119.2017.1335208 |
|
PLoS One |
Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis Pubmed: 30939166 |
|
МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ |
|
|
International Journal of Molecular Sciences |
A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor Pubmed: 31052424 |
|
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY |
MT 1‐MMP evaluation in neointimal hyperplasia in the late follow‐up after prosthesis implantation |
|
Journal of Plastic, Reconstructive & Aesthetic Surgery |
Intraluminal fluid infusion in a rat jejunum ischemia/reperfusion model is associated with improved tissue perfusion and less mucosal damage Pubmed: 31734236 |
|
Bone Research |
Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis Pubmed: 32047705 |
|
Scientific Reports |
Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722 |
|
JOURNAL OF ETHNOPHARMACOLOGY |
Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling … Pubmed: 32745509 |
|
Food Funct |
Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice 33890588 |
|
Phytomedicine |
Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK … Pubmed:35660352 |
|
Journal of Immunology Research |
Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes Pubmed:35340587 |
|
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