SKU:SEC206Mu
ELISA Kit for Histone Deacetylase 1 (HDAC1)
ELISA Kit for Histone Deacetylase 1 (HDAC1)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEC206Mu
Organism Species
Mus musculus (Mouse).
Sample Type
tissue homogenates, cell lysates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.059ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Histone Deacetylase 1 (HDAC1).
No significant cross-reactivity or interference between Histone Deacetylase 1 (HDAC1) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Deacetylase 1 (HDAC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Deacetylase 1 (HDAC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Magazine | Citations |
Journal of Integrative Medicine | Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase Pubmed:25609369 |
Tzu Chi Medical Journal | Effects of tetrandrine and caffeine on cell viability and expression of mammalian target of rapamycin, phosphatase and tensin homolog, histone deacetylase 1, and histone acetyltransferase in glioma cells Science: Article |
Restor Neurol Neurosci | Uridine protects against hypoxic-ischemic brain injury by reducing histone deacetylase activity in neonatal rats PubMed: 26410212 |
Pharmacol Biochem Behav | Lacosamide reduces HDAC levels in the brain and improves memory: Potential for treatment of Alzheimer's disease PubMed: 25931268 |
网页打不开 | Effects of caffeine on cell viability and activity of histone deacetylase 1 and histone acetyltransferase in glioma cells S1016319016300295 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPC206Mu01 | Recombinant Histone Deacetylase 1 (HDAC1) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC206Mu02 | Recombinant Histone Deacetylase 1 (HDAC1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC206Mu01 | Polyclonal Antibody to Histone Deacetylase 1 (HDAC1) | WB; IHC; ICC; IP. |
PAC206Mu02 | Polyclonal Antibody to Histone Deacetylase 1 (HDAC1) | WB; IHC; ICC; IP. |
SEC206Mu | ELISA Kit for Histone Deacetylase 1 (HDAC1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC206Mu | Magnetic Luminex Assay Kit for Histone Deacetylase 1 (HDAC1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |