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SKU:SEB477Mu
ELISA Kit for Matrix Gla Protein (MGP)
ELISA Kit for Matrix Gla Protein (MGP)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEB477Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
0.47-30ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.19ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Matrix Gla Protein (MGP).
No significant cross-reactivity or interference between Matrix Gla Protein (MGP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Matrix Gla Protein (MGP) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Gla Protein (MGP) in samples.
|
Matrix |
Recovery range (%) |
Average(%) |
|
serum(n=5) |
95-103 |
99 |
|
EDTA plasma(n=5) |
83-103 |
98 |
|
heparin plasma(n=5) |
99-105 |
102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Gla Protein (MGP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Gla Protein (MGP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Gla Protein (MGP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
94-101% |
80-91% |
84-93% |
91-105% |
|
EDTA plasma(n=5) |
87-97% |
80-90% |
78-103% |
78-95% |
|
heparin plasma(n=5) |
83-101% |
78-98% |
78-101% |
87-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|
Reagents |
Quantity |
Reagents |
Quantity |
|
Pre-coated, ready to use 96-well strip plate |
1 |
Plate sealer for 96 wells |
4 |
|
Standard |
2 |
Standard Diluent |
1×20mL |
|
Detection Reagent A |
1×120µL |
Assay Diluent A |
1×12mL |
|
Detection Reagent B |
1×120µL |
Assay Diluent B |
1×12mL |
|
TMB Substrate |
1×9mL |
Stop Solution |
1×6mL |
|
Wash Buffer (30 × concentrate) |
1×20mL |
Instruction manual |
1 |
Assay procedure summary
-
Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|
Magazine |
Citations |
|
Nephrology Dialysis Transplantation |
Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells PubMed: PMC3611891 |
|
Maturitas. |
Bone metabolism regulators and arterial stiffness in postmenopausal women Pubmed: 23916080 |
|
The Egyptian Rheumatologist |
Osteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activity ScienceDirect: S1110116414000076 |
|
Toxicology Reports |
Canola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice Researchgate:Source |
|
Lipids in Health and Disease |
Markers of increased cardiovascular risk in patients with chronic kidney disease Biomedcentral:Source |
|
Human & Veterinary Medicine |
The behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veins Hvm:Source |
|
Pol Arch Med Wewn |
Effect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5 PubMed: 26176325 |
|
Lipids Health Dis. |
Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary study pmc:PMC4739105 |
|
Kidney Blood Press Res. |
Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney Disease Pubmed:27100101 |
|
Archives of Oral Biology |
Matrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculus pubmed:27348051 |
|
Cell Death & Disease |
Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis Pubmed:29880866 |
|
Urology |
High concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla protein Pubmed:29935264 |
|
Clinica Chimica Acta |
Serum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseases Pubmed: 30597137 |
|
Biological Trace Element Research |
Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients Pubmed: 30847765 |
|
Renal Failure |
Relationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patients Pubmed: 31538831 |
|
Heliyon |
Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4 Pubmed: 32322728 |
|
Catalog No. |
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Applications (RESEARCH USE ONLY!) |
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PAB477Mu02 |
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