• ELISA Kit for Heat Shock Protein 70 (HSP70)
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SEA873Ga

ELISA Kit for Heat Shock Protein 70 (HSP70)

ELISA Kit for Heat Shock Protein 70 (HSP70)

$684.00 USD
$684.00 USD
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UOM
Sensitivity
Detection range

Enzyme-linked immunosorbent assay for Antigen Detection.

Product No.

SEA873Ga

Organism Species

Chicken (Gallus).

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Test Method

Double-antibody Sandwich

Assay Length

3h

Detection Range

1.56-100ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.55ng/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

Specificity

This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 70 (HSP70).
No significant cross-reactivity or interference between Heat Shock Protein 70 (HSP70) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Heat Shock Protein 70 (HSP70) and the recovery rates were calculated by comparing the measured value to the expected amount of Heat Shock Protein 70 (HSP70) in samples.

Matrix

Recovery range (%)

Average(%)

serum(n=5)

98-105

102

EDTA plasma(n=5)

96-103

101

heparin plasma(n=5)

79-105

81

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heat Shock Protein 70 (HSP70) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

90-101%

79-93%

92-101%

89-97%

EDTA plasma(n=5)

94-102%

95-103%

80-92%

86-103%

heparin plasma(n=5)

96-105%

79-105%

92-101%

88-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents

Quantity

Reagents

Quantity

Pre-coated, ready to use 96-well strip plate

1

Plate sealer for 96 wells

4

Standard

2

Standard Diluent

1×20mL

Detection Reagent A

1×120µL

Assay Diluent A

1×12mL

Detection Reagent B

1×120µL

Assay Diluent B

1×12mL

TMB Substrate

1×9mL

Stop Solution

1×6mL

Wash Buffer (30 × concentrate)

1×20mL

Instruction manual

1

Assay procedure summary

  1. Prepare all reagents, samples and standards;
    2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
    4. Aspirate and wash 3 times;
    5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
    6. Aspirate and wash 5 times;
    7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
    8. Add 50µL Stop Solution. Read at 450nm immediately.

Magazine

Citations

Journal of Molecular Medicine

Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy SpringerLink: b8738j3571287425

Journal of Neuroinflammation

Neuroinflammation in autism spectrum disorders PubMed: PMC3549857

Eur Arch Psychiatry Clin Neurosci.

Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder Pubmed: 23455589

Arch Bronconeumol

Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue PubMed: 26055465

Arch Bronconeumol

Heat Shock Proteins, PubMed: 26055465

Animal Science Journal

The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells PubMed: 26467738

Transplant Proc

L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients PubMed: 26518955

Plos One

Long-Term Effects of Induced Hypothermia on Local and Systemic Inflammation-Results from a Porcine Long-Term Trauma Model Pubmed:27144532

J Pak Med Assoc.

Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine mnstemps:33-1429777310

Egyptian Poultry Science Journal

STUDY THE RELATIONSHIP BETWEEN SELENIUM AND HEAT SHOCK PROTEINS UNDER HEAT STRESS FOR LOCAL SINAI CHICKENS STRAIN abstract:3d115259031

Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load S0144860916301935

Animal Science Journal

Prokaryotic expression of chicken interferon-γ fusion protein and its effect on expression ofpoultry heat shock protein 70 under heat stress. pubmed:27753175

Pharmacol Rep

High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver. pubmed:28531762

Biomed Pharmacother.

SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. pubmed:28122311

Immunobiology

Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer pubmed:29111316

Egyptian Poultry Science Journal

EFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS. 125788660

Biomedicine & Pharmacotherapy

SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death DOI:10.1016/j.biopha.2017.01.087

Egyptian Poultry Science Journal

IMPACT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON PRODUCTIVE PERFORMANCE, IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF BROILER CHICKENS UNDER HEAT-STRESS CONDITION. ISSN: 1110-5623

Aquaculture

Physiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems 10.1016/j.aquaculture.2017.11.030

Journal of dairy research

Detection of 70 kDa heat shock protein in the saliva of dairy cows. pubmed:28625184

Journal of animal science

Reducing rumen starch fermentation of wheat with three percent sodium hydroxide has the potential to ameliorate the effect of heat stress in grain-fed wethers pubmed:29293755

Biochemistry and Cell Biology

Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy Pubmed:29365285

Egyptian Poultry Science Journal

THE EFFECT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF LAYING HENS UNDER HEAT … article_5570_5c06907ac09a7724a58d2b796fe91aba.pdf

Egyptian Poultry Science Journal

EFFECT OF MITIGATION OF HEAT STRESS BY EARLY HEATE ACCLIMATION AND GLUTAMINE INJECTION ON SOME PHYSIOLOGICAL MEASUREMENTS … :

FASEB Journal

Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922

Journal of Biological Sciences

Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker

Biological Procedures Online

Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells

Journal of Biological Sciences

Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer

博士论文

PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE

environmental science and pollution research

Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains Pubmed: 31468353

ACTA TROPICA

Oxidative stress mediated apoptotic potential of mefloquine on experimental trichinellosis Pubmed: 33221280

BioMed Research International

Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488

Slovak Journal of Animal Science

EFFECTS OF HEAT STRESS ON PHYSIOLOGICAL PARAMETERS AND SERUM CONCENTRATION OF HSP70 IN INDIGENOUS BREEDS OF SHEEP IN NIGERIA

博士论文

Effect of organic acid blend and prebiotic on growth performance gut histomorphometry, tibia bone characteristics, reactive oxygen species, microbial count and …

Translational Medicine Communications

Concurrent high-intensity aerobic and resistance exercise modulates systemic release of alarmins (HMGB1, S100A8/A9, HSP70) and inflammatory …

Physiological impacts of suspended solids in recirculating aquaculture

Clin Oral Investig

Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces 34091792

Journal of Thermal Biology

Effects of L-proline on cellular responses of hen erythrocytes subjected to thermal stress 33627283

J Korean Neurosurg Soc

The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model 34376039

PLoS One

Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats Pubmed:35421180

Med Science

Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood


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