ELISA Kit for Cytokeratin 18 (CK18)

Enzyme-linked immunosorbent assay for Antigen Detection.

Product No.

SEB231Po

Organism Species

Sus scrofa; Porcine (Pig).

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Test Method

Double-antibody Sandwich

Assay Length

3h

Detection Range

0.156-10ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.058ng/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

Specificity

This assay has high sensitivity and excellent specificity for detection of Cytokeratin 18 (CK18).
No significant cross-reactivity or interference between Cytokeratin 18 (CK18) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cytokeratin 18 (CK18) and the recovery rates were calculated by comparing the measured value to the expected amount of Cytokeratin 18 (CK18) in samples.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 18 (CK18) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 18 (CK18) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytokeratin 18 (CK18) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Assay procedure summary

1. Prepare all reagents, samples and standards;
   2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
   3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
   4. Aspirate and wash 3 times;
   5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
   6. Aspirate and wash 5 times;
   7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
   8. Add 50µL Stop Solution. Read at 450nm immediately.