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SKU:SEB336Mu
ELISA Kit for Cluster of Differentiation 59 (CD59)
ELISA Kit for Cluster of Differentiation 59 (CD59)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEB336Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, urine and other biological fluids.
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
62.50-4000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 25.5pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Cluster of Differentiation 59 (CD59).
No significant cross-reactivity or interference between Cluster of Differentiation 59 (CD59) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cluster of Differentiation 59 (CD59) and the recovery rates were calculated by comparing the measured value to the expected amount of Cluster of Differentiation 59 (CD59) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 79-99 | 91 |
| heparin plasma(n=5) | 97-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster of Differentiation 59 (CD59) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster of Differentiation 59 (CD59) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cluster of Differentiation 59 (CD59) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 81-98% | 82-95% | 91-99% |
| EDTA plasma(n=5) | 97-105% | 88-102% | 82-91% | 81-105% |
| heparin plasma(n=5) | 96-103% | 78-101% | 99-105% | 78-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| Sci Rep | Soluble CD59 is a Novel Biomarker for the Prediction of Obstructive Chronic Lung Allograft Dysfunction After Lung Transplantation Pubmed:27215188 |
| J Periodontal Res | Salivary levels of last generation specific pro©\resolving lipid mediators (SPMs)(protectin and maresin) in patients with cardiovascular and periodontal disease: A case ¡ 33650687 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB336Mu02 | Recombinant Cluster of Differentiation 59 (CD59) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB336Mu01 | Recombinant Cluster of Differentiation 59 (CD59) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB336Mu01 | Polyclonal Antibody to Cluster of Differentiation 59 (CD59) | WB; IHC; ICC; IP. |
| PAB336Mu02 | Polyclonal Antibody to Cluster of Differentiation 59 (CD59) | WB; IHC; ICC; IP. |
| LAB336Mu71 | Biotin-Linked Polyclonal Antibody to Cluster of Differentiation 59 (CD59) | WB; IHC; ICC. |
| SEB336Mu | ELISA Kit for Cluster of Differentiation 59 (CD59) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB336Mu | Magnetic Luminex Assay Kit for Cluster of Differentiation 59 (CD59) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB336Mu01 | ELISA Kit DIY Materials for Cluster of Differentiation 59 (CD59) | Main materials for "Do It(ELISA Kit) Yourself" |
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