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SKU:SEC158Ra
ELISA Kit for Collagen Type XII (COL12)
ELISA Kit for Collagen Type XII (COL12)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEC158Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma and other biological fluids.
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
0.312-20ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.134ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Collagen Type XII (COL12).
No significant cross-reactivity or interference between Collagen Type XII (COL12) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Collagen Type XII (COL12) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type XII (COL12) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-96 | 86 |
| EDTA plasma(n=5) | 94-102 | 97 |
| heparin plasma(n=5) | 95-102 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type XII (COL12) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type XII (COL12) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type XII (COL12) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-105% | 78-97% | 93-101% | 78-104% |
| EDTA plasma(n=5) | 94-102% | 80-105% | 83-93% | 83-104% |
| heparin plasma(n=5) | 97-105% | 79-98% | 96-105% | 87-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC158Ra01 | Recombinant Collagen Type XII (COL12) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC158Ra01 | Polyclonal Antibody to Collagen Type XII (COL12) | WB; IHC; ICC; IP. |
| MAC158Ra21 | Monoclonal Antibody to Collagen Type XII (COL12) | WB; IHC; ICC; IP. |
| SEC158Ra | ELISA Kit for Collagen Type XII (COL12) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC158Ra | Magnetic Luminex Assay Kit for Collagen Type XII (COL12) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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