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SKU:SCB281Hu
CLIA Kit for Tissue Polypeptide Specific Antigen (TPS)
CLIA Kit for Tissue Polypeptide Specific Antigen (TPS)
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Chemiluminescent immunoassay for Antigen Detection.
Product No.
SCB281Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma and other biological fluids.
Test Method
Double-antibody Sandwich
Assay Length
2h, 40min
Detection Range
27.4-20,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 10.5pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Tissue Polypeptide Specific Antigen (TPS).
No significant cross-reactivity or interference between Tissue Polypeptide Specific Antigen (TPS) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Tissue Polypeptide Specific Antigen (TPS) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Polypeptide Specific Antigen (TPS) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 83-95 | 91 |
| heparin plasma(n=5) | 95-103 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Polypeptide Specific Antigen (TPS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Polypeptide Specific Antigen (TPS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Polypeptide Specific Antigen (TPS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-104% | 80-94% | 93-103% | 91-105% |
| EDTA plasma(n=5) | 82-99% | 96-104% | 78-101% | 97-104% |
| heparin plasma(n=5) | 78-92% | 93-101% | 89-103% | 86-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Substrate A | 1×10mL | Substrate B | 1×2mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
| Magazine | Citations |
| Pancreatology | Diagnostic Validity of Serum Macrophage Inhibitor Cytokine and Tissue Polypeptide-Specific Antigen in Pancreatobiliary Diseases Karger: 328963 |
| Revista do Instituto de Medicina Tropical de São Paulo | Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients Pubmed: 30365640 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| SCB281Hu | CLIA Kit for Tissue Polypeptide Specific Antigen (TPS) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB281Hu | Magnetic Luminex Assay Kit for Tissue Polypeptide Specific Antigen (TPS) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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