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SKU:WEC026Ra
Wide-range ELISA Kit for Salusin Beta (SALb)
Wide-range ELISA Kit for Salusin Beta (SALb)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
WEC026Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
3h
Detection Range
24.69-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 9.25pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Wide-range Salusin Beta (SALb).
No significant cross-reactivity or interference between Wide-range Salusin Beta (SALb) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Wide-range Salusin Beta (SALb) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Salusin Beta (SALb) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-98 | 93 |
| EDTA plasma(n=5) | 88-101 | 96 |
| heparin plasma(n=5) | 79-92 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Salusin Beta (SALb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Salusin Beta (SALb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Salusin Beta (SALb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-104% | 95-105% | 80-95% | 86-94% |
| EDTA plasma(n=5) | 83-101% | 78-102% | 91-98% | 92-101% |
| heparin plasma(n=5) | 78-93% | 79-90% | 78-97% | 89-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
| Magazine | Citations |
| Journal of Maternal-Fetal and Neonatal Medicine | Serum levels of apelin, salusin-alpha, and salusin-beta in normal pregnancy and preeclampsia Informa: 14767058.2012.660221 |
| Peptides | The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass PubMed: 22884920 |
| Department of Medical Biochemistry and Clinical Biochemistry | Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows ScienceDirect: S0196978113000533 |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. PubMed: 23178004 |
| Biotech Histochem. | Serum concentration and kidney expression of salusin-α and salusin-β in rats with metabolic syndrome induced by fructose. PubMed: 23244237 |
| Gynecological Endocrinology, | Salusins, newly identified regulators of hemodynamics and mitogenesis, increase in polycystic ovarian syndrome. PubMed: 22827297 |
| Peptides | Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus ScienceDirect: S0196978113003100 |
| Biotechnic & Histochemistry | Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome Com:Source |
| Med Sci Monit. | Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome Pubmed:Pmc4136931 |
| Acta Physiologica | Superoxide anions involved in sympathoexcitation and pressor effects of salusin-β in paraventricular nucleus in hypertensive rats Pubmed:24304512 |
| Pediatr Nephrol. | Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? Pubmed:25245503 |
| European Journal of Dermatology | Serum salusin-α and salusin-β levels in patients with psoriasis PubMed: 26055619 |
| J Investig Med | Serum Salusin-β Levels Are Associated With the Presence and Severity of Coronary Artery Disease PubMed: 25730454 |
| Genet Test Mol Biomarkers. | Serum Salusin-β Levels Are Correlated with Slow Coronary Flow. Pubmed:27227272 |
| Sci Rep. | Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. pubmed:28230187 |
| Cell Death Dis. | Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy pubmed:28333148 |
| Oxidative Medicine and Cellular Longevity | Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma 10.1155:2017 |
| International Journal Of Cardiology | Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus Pubmed: 30581101 |
| Multiple Sclerosis and Related Disorders | Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis Pubmed: 30738876 |
| cardiology in the young | Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity Pubmed: 31486354 |
| Redox Biology | Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway Pubmed: 31884071 |
| Journal of international medical research | Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis Pubmed: 32054363 |
| cardiovascular drugs and therapy | A TOR2A Gene Product: Salusin-β Contributes to Attenuated Vasodilatation of Spontaneously Hypertensive Rats. Pubmed: 32458319 |
| Serum level of salusin β as an indicator of metabolic disorders in Acute Lymphoblastic Leucaemia and Wilms Tumour survivors | |
| Mol Med Rep | Salusin‑β participates in high glucose‑induced HK‑2 cell ferroptosis in a Nrf‑2‑dependent manner 34296310 |
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