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ELISA Kit for Von Willebrand Factor Cleaving Protease (vWFCP)
ELISA Kit for Von Willebrand Factor Cleaving Protease (vWFCP)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEA950Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
31.2-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 12.2pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Von Willebrand Factor Cleaving Protease (vWFCP).
No significant cross-reactivity or interference between Von Willebrand Factor Cleaving Protease (vWFCP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Von Willebrand Factor Cleaving Protease (vWFCP) and the recovery rates were calculated by comparing the measured value to the expected amount of Von Willebrand Factor Cleaving Protease (vWFCP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-101 | 96 |
| EDTA plasma(n=5) | 86-104 | 93 |
| heparin plasma(n=5) | 85-92 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Willebrand Factor Cleaving Protease (vWFCP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Willebrand Factor Cleaving Protease (vWFCP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Von Willebrand Factor Cleaving Protease (vWFCP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-91% | 87-101% | 88-102% | 88-101% |
| EDTA plasma(n=5) | 94-101% | 93-101% | 88-101% | 92-101% |
| heparin plasma(n=5) | 89-97% | 86-94% | 84-99% | 85-92% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| 33 | Neutrophil Protease Cleavage of Von Willebrand Factor in Glomeruli - An Anti-thrombotic Mechanism in the Kidney. pubmed:28139439 |
| Experimental and Therapeutic Medicine | Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with … |
| Scientific Reports | Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| SEA950Mu | ELISA Kit for Von Willebrand Factor Cleaving Protease (vWFCP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA950Mu | Magnetic Luminex Assay Kit for Von Willebrand Factor Cleaving Protease (vWFCP) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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