Cloud-clone
SKU:RPL917Ra01
Recombinant Semaphorin 3A (SEMA3A)
Recombinant Semaphorin 3A (SEMA3A)
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Prokaryotic expression
Product No.
RPL917Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
17.6kDa
Accurate Molecular Mass
17kDa(Analysis of differences refer to the manual)
Residues & Tags
Arg31~Pro150 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
8.9
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Arthritis Research & Therapy | Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus Biomed: ar3881 |
| journal of invastigative dermatology | Cathelicidin LL-37 Induces Semaphorin 3A Expression in Human Epidermal Keratinocytes: Implications for Possible Application to Pruritus PubMed: 26121211 |
| Neurology | Neurofilament light chain level is a weak risk factor for the development of MS. pubmed:27521440 |
| Development | miR-126-5p promotes retinal endothelial cell survival through SetD5 regulatio in neurons pubmed:29180574 |
| A Preliminary Study of the Effect of Semaphorin 3A and Acitretin on the Proliferation, Migration, and Apoptosis of HaCaT Cells | |
| Life Sciences | Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway. Pubmed: 32603817 |
| ACS NANO | Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling Pubmed: 32413258 |
| PLoS One | Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes 33905439 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPL917Ra01 | Recombinant Semaphorin 3A (SEMA3A) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL917Ra01 | Polyclonal Antibody to Semaphorin 3A (SEMA3A) | WB; IHC; ICC; IP. |
| MAL917Ra21 | Monoclonal Antibody to Semaphorin 3A (SEMA3A) | WB; IHC; ICC; IP. |
| SEL917Ra | ELISA Kit for Semaphorin 3A (SEMA3A) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LML917Ra | Magnetic Luminex Assay Kit for Semaphorin 3A (SEMA3A) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
