Cloud-clone
SKU:RPN048Hu01
Recombinant High Temperature Requirement Factor A4 (HTRA4)
Recombinant High Temperature Requirement Factor A4 (HTRA4)
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Prokaryotic expression
Product No.
RPN048Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
20.9kDa
Accurate Molecular Mass
21kDa(Analysis of differences refer to the manual)
Residues & Tags
Gly202~Leu362 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
4.5
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Placenta | Upregulation of HtrA4 in the placentas of patients with severe pre-eclampsia PubMed: 22964307 |
| J Clin Endocrinol Metab | Human HtrA4 Expression Is Restricted to the Placenta, Is Significantly Up-Regulated in Early-Onset Preeclampsia, and High Levels of HtrA4 Cause Endothelial Dysfunction - See more at: http://press.endocrine.org/doi/abs/10.1210/jc.2014-3969#sthash.XcUOSx2Z.dpuf PubMed: 25946029 |
| Journal of Biomolecular Screening | HtrA3 Isoform-Specific ELISAs for Early Detection of Preeclampsia. pubmed:27932697 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPN048Hu01 | Recombinant High Temperature Requirement Factor A4 (HTRA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAN048Hu01 | Polyclonal Antibody to High Temperature Requirement Factor A4 (HTRA4) | WB; IHC; ICC; IP. |
| MAN048Hu21 | Monoclonal Antibody to High Temperature Requirement Factor A4 (HTRA4) | WB; IHC; ICC; IP. |
| SEN048Hu | ELISA Kit for High Temperature Requirement Factor A4 (HTRA4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCN048Hu | CLIA Kit for High Temperature Requirement Factor A4 (HTRA4) | Chemiluminescent immunoassay for Antigen Detection. |
| LMN048Hu | Magnetic Luminex Assay Kit for High Temperature Requirement Factor A4 (HTRA4) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
