Cloud-clone
SKU:RPA072Mu01
Recombinant Defensin Beta 2 (DEFb2)
Recombinant Defensin Beta 2 (DEFb2)
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Prokaryotic expression
Product No.
RPA072Mu01
Organism Species
Mus musculus (Mouse).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
35.5kDa
Accurate Molecular Mass
38kDa(Analysis of differences refer to the manual)
Residues & Tags
Ala21~Lys71 with N-terminal His and GST Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
8.4
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Livestock Science | The effect of Lactobacillus on the expression of porcine β-defensin-2 in the digestive tract of piglets ScienceDirect: S1871141311000126 |
| Appl Environ Microbiol. | Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line Asm: Source |
| Plos Pathogens | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in?Pseudomonas aeruginosa?Keratitis PubMed: PMC3715414 |
| British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese Pubmed: 24124699 |
| University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis Waikato:Source |
| Vaccine | On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment Pubmed:25444792 |
| International Journal of Dairy Technology | Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula Doi: 10.1111 |
| Molecular Nutrition Food Research | Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. Pubmed:26991948 |
| Respiratory Research | Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner. pubmed:27655266 |
| Key Engineering Materials | The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture. 16629795 |
| Scientific Reports | Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique Pubmed:29374283 |
| Frontiers in Microbiology | Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense Pubmed:29997590 |
| Journal of Dermatological Science | Glyoxal-induced exacerbation of pruritus and dermatitis is associated with staphylococcus aureus colonization in the skin of a rat model of atopic dermatitis Pubmed:29496360 |
| International Immunopharmacology | Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase … Pubmed: 30342347 |
| 拉脱维亚科学院院刊 | Influence of Antibiotic-Impregnated Biomaterials on Inflammatory Cytokines |
| Veterinary Research | Bovine fetal mesenchymal stem cells exert antiproliferative effect against mastitis causing pathogen Staphylococcus aureus Pubmed: 30975214 |
| Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients |
| Veterinary Research | Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection Pubmed: 31221216 |
| Animals | Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport 33375218 |
| Sci Rep | Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus 34362937 |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … Pubmed:35687771 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA072Mu02 | Recombinant Defensin Beta 2 (DEFb2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA072Mu01 | Recombinant Defensin Beta 2 (DEFb2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA072Mu02 | Polyclonal Antibody to Defensin Beta 2 (DEFb2) | WB; IHC; ICC; IP. |
| PAA072Mu01 | Polyclonal Antibody to Defensin Beta 2 (DEFb2) | WB; IHC; ICC; IP. |
| SEA072Mu | ELISA Kit for Defensin Beta 2 (DEFb2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA072Mu | Magnetic Luminex Assay Kit for Defensin Beta 2 (DEFb2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
