Cloud-clone
SKU:RPA109Hu01
Recombinant Creatine Kinase, Muscle (CKM)
Recombinant Creatine Kinase, Muscle (CKM)
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Prokaryotic expression
Product No.
RPA109Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Cytoplasm
Predicted Molecular Mass
41.5kDa
Accurate Molecular Mass
41kDa(Analysis of differences refer to the manual)
Residues & Tags
Lys11~Leu367 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
7.3
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Journal of Inflammation | Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgery PubMed: 22583529 |
| PLoS One | Extraocular Muscle Characteristics Related to Myasthenia Gravis Susceptibility PubMed: PMC3568149 |
| Kidney Int. | Fructokinase activity mediates dehydration-induced renal injury Pubmed: 24336030 |
| Methylxanthines and cardiotoxicity | Methylxanthine induced cardiotoxicity and its mechanisms: An experimental study 230b:34bf146150b42971d76dda5e8329bc5f2f0b.pdf |
| Molecular Medicine Reports | Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473 |
| Toxicological Sciences | Proteomic and Metabolomic Profiling Identify Plasma Biomarkers for Exposure to Ultra-low Levels of Carfentanil Pubmed: 30321427 |
| Clinical & Translational Oncology | The effects of human sera conditioned by high-intensity exercise sessions and training on the tumorigenic potential of cancer cells Pubmed: 32447643 |
