Cloud-clone
SKU:RPA126Fi01
Recombinant TEK Tyrosine Kinase, Endothelial (Tie2)
Recombinant TEK Tyrosine Kinase, Endothelial (Tie2)
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Prokaryotic expression
Product No.
RPA126Fi01
Organism Species
Danio rerio (Zebrafish).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Membrane, Cytoplasm
Predicted Molecular Mass
61.3kDa
Accurate Molecular Mass
61kDa(Analysis of differences refer to the manual)
Residues & Tags
Val22~Ala312 with N-terminal His and GST Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
6.5
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Anesthesiology | Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … Pubmed:28968277 |
| British Journal of Anaesthesia | Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats 10.1016:j.bja.2018.05.049 |
| Intensive Care Med Exp | The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats 33997943 |
| Catalog No. | Related products for research use of Danio rerio (Zebrafish) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA126Fi01 | Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA126Fi01 | Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) | WB; IHC; ICC; IP. |
