Cloud-clone
SKU:RPA126Ra01
Recombinant TEK Tyrosine Kinase, Endothelial (Tie2)
Recombinant TEK Tyrosine Kinase, Endothelial (Tie2)
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Prokaryotic expression
Product No.
RPA126Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
63.6kDa
Accurate Molecular Mass
64kDa(Analysis of differences refer to the manual)
Residues & Tags
Cys44~Glu341 with N-terminal His and GST Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
8.0
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Anesthesiology | Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … Pubmed:28968277 |
| British Journal of Anaesthesia | Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats 10.1016:j.bja.2018.05.049 |
| Intensive Care Med Exp | The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats 33997943 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA126Ra01 | Recombinant TEK Tyrosine Kinase, Endothelial (Tie2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA126Ra01 | Polyclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) | WB; IHC; ICC; IP. |
| MAA126Ra21 | Monoclonal Antibody to TEK Tyrosine Kinase, Endothelial (Tie2) | WB; IHC; ICC; IP. |
| SEA126Ra | ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA126Ra | Magnetic Luminex Assay Kit for TEK Tyrosine Kinase, Endothelial (Tie2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
