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SKU:RPA565Hu01

Recombinant Heparan Sulfate Proteoglycan (HSPG)

Recombinant Heparan Sulfate Proteoglycan (HSPG)

Regular price $360.00 USD
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UOM
Purity
Organism species

Prokaryotic expression

Product No.

RPA565Hu01

Organism Species

Homo sapiens (Human).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane

Predicted Molecular Mass

15.2kDa

Accurate Molecular Mass

23kDa(Analysis of differences refer to the manual)

Residues & Tags

Glu19~Glu144 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 95%

Isoelectric Point

4.7

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

 

SEQUENCE

 

 

 

USAGE

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Journal of Applied Pharmaceutical Science Cytotoxic effects of antiglypican-3 against HepG3 cell line Ebscohost: Source
Journal of Reproductive Immunology Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patients Pubmed: 24661950
European journal of pharmacology Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413
European Journal of Pharmacology Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma Pubmed:25449037
Shock Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance Pubmed:25692257
Biomarkers in Medicine Endothelial glycocalyx layer shedding following lung resection pubmed:27643669
Apmis Circulating syndecans during critical illness. pubmed:28256016
Biomedicine & Pharmacotherapy Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302
Drug Delivery Glioma targeting peptide modified apoferritin nanocage Pubmed:29726297
Anaesthesia Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass Pubmed: 30687934
Animals Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period …
LIFE SCIENCES Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients 33781826
Genet Mol Biol A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate 34042151
Poultry Science Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine?¡­ 33518110
J Gerontol A Biol Sci Med Sci The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … 34423832
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