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SKU:RPA565Ra01
Recombinant Heparan Sulfate Proteoglycan (HSPG)
Recombinant Heparan Sulfate Proteoglycan (HSPG)
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Prokaryotic expression
Product No.
RPA565Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Membrane
Predicted Molecular Mass
17.6kDa
Accurate Molecular Mass
26kDa(Analysis of differences refer to the manual)
Residues & Tags
Glu19~Glu144 with N-terminal His Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
3.9
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Journal of Applied Pharmaceutical Science | Cytotoxic effects of antiglypican-3 against HepG3 cell line Ebscohost: Source |
| Journal of Reproductive Immunology | Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patients Pubmed: 24661950 |
| European journal of pharmacology | Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413 |
| European Journal of Pharmacology | Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma Pubmed:25449037 |
| Shock | Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance Pubmed:25692257 |
| Biomarkers in Medicine | Endothelial glycocalyx layer shedding following lung resection pubmed:27643669 |
| Apmis | Circulating syndecans during critical illness. pubmed:28256016 |
| Biomedicine & Pharmacotherapy | Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302 |
| Drug Delivery | Glioma targeting peptide modified apoferritin nanocage Pubmed:29726297 |
| Anaesthesia | Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass Pubmed: 30687934 |
| Animals | Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period … |
| LIFE SCIENCES | Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients 33781826 |
| Genet Mol Biol | A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate 34042151 |
| Poultry Science | Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine?¡ 33518110 |
| J Gerontol A Biol Sci Med Sci | The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … 34423832 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA565Ra01 | Recombinant Heparan Sulfate Proteoglycan (HSPG) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA565Ra01 | Polyclonal Antibody to Heparan Sulfate Proteoglycan (HSPG) | WB; IHC; ICC; IP. |
| SEA565Ra | ELISA Kit for Heparan Sulfate Proteoglycan (HSPG) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA565Ra | Magnetic Luminex Assay Kit for Heparan Sulfate Proteoglycan (HSPG) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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