Cloud-clone
SKU:RPA631Hu01
Recombinant Midkine (MK)
Recombinant Midkine (MK)
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Prokaryotic expression
Product No.
RPA631Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
20.0kDa
Accurate Molecular Mass
20kDa(Analysis of differences refer to the manual)
Residues & Tags
Ala22~Asp143 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
9.4
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Midkine: From Embryogenesis to Pathogenesis and Therapy | Immunochemical Methods for the Detection of Midkine in Samples of Human Origin Springer: Source |
| 33 | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … 10.1016:j.ebiom.2017.11.020 |
| Circulation Journal | Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice pubmed:28781288 |
| EBioMedicine | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … Pubmed:29233575 |
| Journal of Clinical Laboratory Analysis | Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall … Pubmed:29797475 |
| Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese : | |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440 |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells Pubmed: 33206337 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA631Hu02 | Recombinant Midkine (MK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA631Hu01 | Recombinant Midkine (MK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA631Hu01 | Active Midkine (MK) | Cell culture; Activity Assays. |
| PAA631Hu02 | Polyclonal Antibody to Midkine (MK) | WB; IHC; ICC; IP. |
| PAA631Hu01 | Polyclonal Antibody to Midkine (MK) | WB; IHC; ICC; IP. |
| LAA631Hu71 | Biotin-Linked Polyclonal Antibody to Midkine (MK) | WB; IHC; ICC. |
| LAA631Hu81 | FITC-Linked Polyclonal Antibody to Midkine (MK) | WB; IHC; ICC; IF. |
| MAA631Hu22 | Monoclonal Antibody to Midkine (MK) | WB; IHC; ICC; IP. |
| MAA631Hu21 | Monoclonal Antibody to Midkine (MK) | WB; IHC; ICC; IP. |
| SEA631Hu | ELISA Kit for Midkine (MK) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA631Hu | Magnetic Luminex Assay Kit for Midkine (MK) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA631Hu01 | ELISA Kit DIY Materials for Midkine (MK) | Main materials for "Do It (ELISA Kit) Yourself". |
