Cloud-clone
SKU:RPA928Ra01
Recombinant Tumor Protein p53 (P53)
Recombinant Tumor Protein p53 (P53)
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Prokaryotic expression
Product No.
RPA928Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Nucleus, Cytoplasm, Endoplasmic reticulum lumen
Predicted Molecular Mass
33.7kDa
Accurate Molecular Mass
36kDa(Analysis of differences refer to the manual)
Residues & Tags
Pro90~Ala354 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
5.9
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Anticancer Research | Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines PubMed: 23155233 |
| Microscopy Research and Technique | Therapeutic role of curcumin in oxidative DNA damage caused by formaldehyde PubMed: 25761397 |
| J Nutr Biochem | Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice PubMed: 26344777 |
| Biochemical and Biophysical Research Communications | Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes Pubmed: 31331641 |
| Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA928Ra01 | Recombinant Tumor Protein p53 (P53) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA928Ra01 | Active Tumor Protein p53 (P53) | Cell culture; Activity Assays. |
| PAA928Ra01 | Polyclonal Antibody to Tumor Protein p53 (P53) | WB,ICC/IF |
| MAA928Ra22 | Monoclonal Antibody to Tumor Protein p53 (P53) | WB; IHC; ICC; IP. |
| MAA928Ra21 | Monoclonal Antibody to Tumor Protein p53 (P53) | WB; IHC; ICC; IP. |
| SEA928Ra | ELISA Kit for Tumor Protein p53 (P53) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA928Ra | Magnetic Luminex Assay Kit for Tumor Protein p53 (P53) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
