Cloud-clone
SKU:RPA936Mu01
Recombinant Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)
Recombinant Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)
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Prokaryotic expression
Product No.
RPA936Mu01
Organism Species
Mus musculus (Mouse).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Cytoplasm
Predicted Molecular Mass
31.9kDa
Accurate Molecular Mass
27kDa(Analysis of differences refer to the manual)
Residues & Tags
Ile312~Ala564 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
7.8
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Endocrinology | In utero exposure to the endocrine disruptor di-(2-ethylhexyl) phthalate induces long-term changes in gene expression in the adult male adrenal gland Pubmed: 24564399 |
| Cellular Physiology and Biochemistry | Overexpression of PCK1 Gene Antagonizes Hepatocellular Carcinoma Through the Activation of Gluconeogenesis and Suppression of Glycolysis Pathways Pubmed:29768256 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA936Mu01 | Recombinant Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA936Mu02 | Recombinant Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA936Mu02 | Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) | WB; IHC; ICC; IP. |
| PAA936Mu01 | Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) | WB; IHC; ICC; IP. |
| SEA936Mu | ELISA Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA936Mu | Magnetic Luminex Assay Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
