Cloud-clone
SKU:RPA937Ra01
Recombinant Nephrin (NPHN)
Recombinant Nephrin (NPHN)
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Prokaryotic expression
Product No.
RPA937Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Membrane
Predicted Molecular Mass
38.4kDa
Accurate Molecular Mass
41kDa(Analysis of differences refer to the manual)
Residues & Tags
Pro39~Gly106 with N-terminal His and GST Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
9.8
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Yonsei medical journal | The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia. pubmed:28120572 |
| Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788 |
| British Journal of Haematology | Renal protection by atorvastatin in a murine model of sickle cell nephropathy Pubmed:29527679 |
| Journal of Clinical Medicine | Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease Pubmed: 32961903 |
| Disease Markers | Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study 34707724 |
| Biomed Rep | Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs Pubmed:35620307 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA937Ra01 | Recombinant Nephrin (NPHN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA937Ra01 | Polyclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
| MAA937Ra21 | Monoclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
| SEA937Ra | ELISA Kit for Nephrin (NPHN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA937Ra | Magnetic Luminex Assay Kit for Nephrin (NPHN) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA937Ra01 | ELISA Kit DIY Materials for Nephrin (NPHN) | Main materials for "Do It (ELISA Kit) Yourself". |
