Cloud-clone
SKU:RPB317Ra01
Recombinant Perforin 1 (PRF1)
Recombinant Perforin 1 (PRF1)
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Prokaryotic expression
Product No.
RPB317Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Cytoplasmic granule lumen.Secreted.Cell membrane; Multi-pass membrane protein. Endosome lum
Predicted Molecular Mass
29.7kDa
Accurate Molecular Mass
33kDa(Analysis of differences refer to the manual)
Residues & Tags
Ala120~Asp353 (Accession # P35763) with
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
6.3
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in PBS or others.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Diagnostic Microbiology and Infectious Disease | Granzyme B as a diagnostic marker of tuberculosis in patients with and without HIV coinfection Pubmed:26915636 |
| FASEB JOURNAL | The immunoregulatory effects of CD8 T-cell–derived perforin on diet-induced nonalcoholic steatohepatitis Pubmed: 30951375 |
| Theranostics | A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors Pubmed: 31695790 |
| Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Pubmed: 33223223 |
| INVESTIGATIONAL NEW DRUGS | Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors Pubmed: 32772342 |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma 34377237 |
| Nat Biomed Eng | Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy 34873307 |
| J Exp Med | Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion Pubmed:35061012 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB317Ra01 | Recombinant Perforin 1 (PRF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB317Ra01 | Active Perforin 1 (PRF1) | Cell culture; Activity Assays. |
| PAB317Ra01 | Polyclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC; IP. |
| MAB317Ra21 | Monoclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC; IP. |
| SEB317Ra | ELISA Kit for Perforin 1 (PRF1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB317Ra | Magnetic Luminex Assay Kit for Perforin 1 (PRF1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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