Cloud-clone
SKU:RPB360Hu01
Recombinant Acid Sphingomyelinase (ASM)
Recombinant Acid Sphingomyelinase (ASM)
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Prokaryotic expression
Product No.
RPB360Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Lysosome
Predicted Molecular Mass
33.8kDa
Accurate Molecular Mass
30kDa(Analysis of differences refer to the manual)
Residues & Tags
His321~Pro581 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
6.6
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Blood.? | Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD Pubmed:25075126 |
| Journal of Clinical Lipidology | Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes 10.1016/j.jacl.2017.03.007 |
| Diabetes & metabolism | Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696 |
| INFLAMMATION | Acid Sphingomyelinase and Acid ¦Â-Glucosidase 1 Exert Opposite Effects on Interleukin-1¦Â-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes 33665756 |
| Indian J Ophthalmol | Evaluation of sphingolipid metabolism on diabetic retinopathy 34708809 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| EPB360Hu61 | Eukaryotic Acid Sphingomyelinase (ASM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB360Hu01 | Recombinant Acid Sphingomyelinase (ASM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB360Hu61 | Active Acid Sphingomyelinase (ASM) | Cell culture; Activity Assays. |
| PAB360Hu01 | Polyclonal Antibody to Acid Sphingomyelinase (ASM) | WB; IHC; ICC; IP. |
| MAB360Hu22 | Monoclonal Antibody to Acid Sphingomyelinase (ASM) | WB; IHC; ICC; IP. |
| SEB360Hu | ELISA Kit for Acid Sphingomyelinase (ASM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEB360Hu | Mini Samples ELISA Kit for Acid Sphingomyelinase (ASM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB360Hu | Magnetic Luminex Assay Kit for Acid Sphingomyelinase (ASM) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
