Cloud-clone
SKU:RPB831Ra01
Recombinant Hedgehog Homolog, Sonic (SHH)
Recombinant Hedgehog Homolog, Sonic (SHH)
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Prokaryotic expression
Product No.
RPB831Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Membrane
Predicted Molecular Mass
21.2kDa
Accurate Molecular Mass
19kDa(Analysis of differences refer to the manual)
Residues & Tags
Ser237~Arg399 with N-terminal His Tag
Buffer Formulation
100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
8.0
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html |
| Annals of the Rheumatic Diseases | Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis pubmed:28495672 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB831Ra01 | Recombinant Hedgehog Homolog, Sonic (SHH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB831Ra02 | Recombinant Hedgehog Homolog, Sonic (SHH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB831Ra01 | Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) | WB; IHC; ICC; IP. |
| PAB831Ra02 | Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) | WB; IHC; ICC; IP. |
| LAB831Ra71 | Biotin-Linked Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) | WB; IHC; ICC. |
| MAB831Ra21 | Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH) | WB; IHC; ICC; IP. |
| SEB831Ra | ELISA Kit for Hedgehog Homolog, Sonic (SHH) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB831Ra | Magnetic Luminex Assay Kit for Hedgehog Homolog, Sonic (SHH) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB831Ra01 | ELISA Kit DIY Materials for Hedgehog Homolog, Sonic (SHH) | Main materials for "Do It (ELISA Kit) Yourself". |
