Cloud-clone
SKU:RPC228Hu01
Recombinant Occludin (OCLN)
Recombinant Occludin (OCLN)
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Prokaryotic expression
Product No.
RPC228Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Membrane
Predicted Molecular Mass
33.8kDa
Accurate Molecular Mass
42kDa(Analysis of differences refer to the manual)
Residues & Tags
Lys266~Thr522 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
5.5
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Brain Research | HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9 ScienceDirect: S0006899311021408 |
| Plos one | Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and Inflammation PubMed: PMC3688973 |
| Journal of Neurotrauma | A new panel of blood biomarkers for the diagnosis of mild traumatic brain injury/concussion in adults PubMed: 25794137 |
| United States Patent Application | Methods for Diagnosis and Treatment of Concussion or Brain Injury 2016:0103140 |
| Sci Rep. | Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Stroke articles:srep40331 |
| Frontiers in Pharmacology | Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal … Pubmed:29375374 |
| Thorax | Fas activation alters tight junction proteins in acute lung injury Pubmed: 30385692 |
| Antioxidants (Basel). | The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets Pubmed: 31426309 |
| DISEASE MARKERS | Peripheral blood occludin level as a biomarker for preoperative cerebral edema in patients with brain tumors Pubmed: 32884584 |
| Burns Open | Intestinal Permeability in Participants With Thermal Injury: A Case Series From a Prospective, Longitudinal Study (HESTIA) |
| PLoS One | A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and … 34492054 |
| CNS Neuroscience & Therapeutics | Higher serum occludin after successful reperfusion Is associated with early neurological deterioration Pubmed:35338575 |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation … |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC228Hu01 | Recombinant Occludin (OCLN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC228Hu01 | Polyclonal Antibody to Occludin (OCLN) | WB; IHC; ICC; IP. |
| MAC228Hu22 | Monoclonal Antibody to Occludin (OCLN) | WB; IHC; ICC; IP. |
| SEC228Hu | ELISA Kit for Occludin (OCLN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC228Hu | Magnetic Luminex Assay Kit for Occludin (OCLN) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
