Cloud-clone
SKU:RPD294Ra01
Recombinant Cytochrome P450 1A2 (CYP1A2)
Recombinant Cytochrome P450 1A2 (CYP1A2)
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Prokaryotic expression
Product No.
RPD294Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Endoplasmic reticulum lumen
Predicted Molecular Mass
33.0KDa
Accurate Molecular Mass
33kDa(Analysis of differences refer to the manual)
Residues & Tags
Val192~Glu444 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
6.1
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Environmental Monitoring and Assessment | Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytes Pubmed:25471622 |
| The Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4 PubMed: 25733458 |
| Molecular Nutrition Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590 |
| Molecular Nutrition & Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590 |
| Journal of FisheriesSciences. com | Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stress b0407ebde042c4f61b728bc11a586e6c |
| Toxicology | Liver lobe and strain differences in the activity of murine cytochrome p450 enzymes Pubmed:29879457 |
| Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD294Ra01 | Recombinant Cytochrome P450 1A2 (CYP1A2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD294Ra01 | Polyclonal Antibody to Cytochrome P450 1A2 (CYP1A2) | WB; IHC; ICC; IP. |
| MAD294Ra21 | Monoclonal Antibody to Cytochrome P450 1A2 (CYP1A2) | WB; IHC; ICC; IP. |
| SED294Ra | ELISA Kit for Cytochrome P450 1A2 (CYP1A2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD294Ra | Magnetic Luminex Assay Kit for Cytochrome P450 1A2 (CYP1A2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
