Cloud-clone
SKU:RPE038Hu01
Recombinant Neurofilament, Light Polypeptide (NEFL)
Recombinant Neurofilament, Light Polypeptide (NEFL)
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Prokaryotic expression
Product No.
RPE038Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
14.5kDa
Accurate Molecular Mass
15kDa(Analysis of differences refer to the manual)
Residues & Tags
Lys281~Glu396 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
5.9
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Acta Neurologica Scandinavica | Effect of carotid endarterectomy on brain damage markers Pubmed:27126899 |
| Acta Neurologica Scandinavica | Effect of carotid endarterectomy on brain damage markers. pubmed:27126899 |
| journal of neuroimmunology | Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implications Pubmed: 31254929 |
| Cerebellum | Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia 33893614 |
| Medicine (Baltimore) | Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis 34678870 |
| J Clin Med | Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients … Pubmed:35566558 |
