Cloud-clone
SKU:RPE913Hu01
Recombinant Sirtuin 3 (SIRT3)
Recombinant Sirtuin 3 (SIRT3)
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Prokaryotic expression
Product No.
RPE913Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Mitochondrion
Predicted Molecular Mass
31.7kDa
Accurate Molecular Mass
32kDa(Analysis of differences refer to the manual)
Residues & Tags
Gln126~Lys399 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
6.0
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Toxicology Letters | SirT3 regulates diabetogenic effects caused by arsenic: An implication for mitochondrial complex II modification Pubmed: 30385301 |
| International Breastfeeding Journal | Effects of perinatal factors on sirtuin 3, 8-hydroxy-2′-deoxyguanosine, brain-derived neurotrophic factor and serotonin in cord blood and early breast milk: an … Pubmed: 32552911 |
| Antioxidants | The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of?¡ 33396247 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APE913Hu01 | Active Sirtuin 3 (SIRT3) | Cell culture; Activity Assays. |
| RPE913Hu01 | Recombinant Sirtuin 3 (SIRT3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE913Hu01 | Polyclonal Antibody to Sirtuin 3 (SIRT3) | WB; IHC; ICC; IP. |
| LAE913Hu81 | FITC-Linked Polyclonal Antibody to Sirtuin 3 (SIRT3) | WB; IHC; ICC; IF. |
| LAE913Hu71 | Biotin-Linked Polyclonal Antibody to Sirtuin 3 (SIRT3) | WB; IHC; ICC. |
| MAE913Hu21 | Monoclonal Antibody to Sirtuin 3 (SIRT3) | WB,IHC |
| MAE913Hu22 | Monoclonal Antibody to Sirtuin 3 (SIRT3) | WB; IHC |
| MAE913Hu23 | Monoclonal Antibody to Sirtuin 3 (SIRT3) | WB; IHC; ICC; IP. |
| LAE913Hu42 | PE-Linked Monoclonal Antibody to Sirtuin 3 (SIRT3) | WB; IHC; ICC; IF. |
| SEE913Hu | ELISA Kit for Sirtuin 3 (SIRT3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME913Hu | Magnetic Luminex Assay Kit for Sirtuin 3 (SIRT3) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
