Cloud-clone
SKU:RPF396Hu01
Recombinant Spondin 2 (SPON2)
Recombinant Spondin 2 (SPON2)
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Prokaryotic expression
Product No.
RPF396Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
53.1kDa
Accurate Molecular Mass
54kDa(Analysis of differences refer to the manual)
Residues & Tags
Ser119~Val331 with N-terminal His and GST Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
5.6
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| The Journal of Urology | Spondin-2, a secreted extracellular matrix protein, is a novel diagnostic biomarker for prostate cancer Pubmed: 23665271 |
| Oxford Journals | SP136EVALUATION OF SERUM SPONDIN 2 LEVELS IN THE DIFFERENT ETIOLOGIES OF GLOMERULAR DISEASES Content: 30 |
| Nephrology (Carlton) | Evaluation of Serum Spondin 2 Levels in the Different Stages of Type 2 Diabetic Nephropathy PubMed: 25973958 |
| Oncogene | The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulation pubmed:28991232 |
| Endocrinology | Study of human T21 placenta suggests a potential role of mesenchymal spondin-2 in placental vascular development Pubmed: 30715257 |
| Journal of Cellular and Molecular Medicine | Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in mice Pubmed: 32614521 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPF396Hu01 | Recombinant Spondin 2 (SPON2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF396Hu01 | Polyclonal Antibody to Spondin 2 (SPON2) | WB; IHC; ICC; IP. |
| MAF396Hu21 | Monoclonal Antibody to Spondin 2 (SPON2) | WB; IHC; ICC; IP. |
| SEF396Hu | ELISA Kit for Spondin 2 (SPON2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF396Hu | Magnetic Luminex Assay Kit for Spondin 2 (SPON2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
