Cloud-clone
SKU:RPG945Hu01
Recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
Recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
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Prokaryotic expression
Product No.
RPG945Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Cytoplasm, Endoplasmic reticulum lumen, Chromosome
Predicted Molecular Mass
28.2kDa
Accurate Molecular Mass
28kDa(Analysis of differences refer to the manual)
Residues & Tags
Gln2~Ala223 with N-terminal His Tag
Buffer Formulation
100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
6.1
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| J Neurol Sci | Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions PubMed: 26232084 |
| Journal of the Neurological Sciences | Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome Pubmed:26810533 |
| Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy mnstemps:135 |
| Scientific Reports | Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes pubmed:28667290 |
| Intensive Care Medicine | Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability Pubmed:29523900 |
| Earth and Environmental Science | Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury article:10.1088 |
| Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage ISBN:978-952-03-0750-9 | |
| Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness Pubmed: 31896448 |
| Brain Sciences | BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma Pubmed: 32987792 |
