Cloud-clone
SKU:RPB449Bo01
Recombinant Enolase 1 (ENO1)
Recombinant Enolase 1 (ENO1)
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Prokaryotic expression
Product No.
RPB449Bo01
Organism Species
Bos taurus; Bovine (Cattle).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Membrane, Cytoplasm
Predicted Molecular Mass
48.9kDa
Accurate Molecular Mass
48kDa(Analysis of differences refer to the manual)
Residues & Tags
Met1~Lys434 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
6.5
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Analytical Chemistry | Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor PubMed: 20557064 |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules Pubmed:27172385 |
| Journal of Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia Pubmed:29654921 |
| BioMed Research International | The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis Pubmed: 33062681 |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212 |
| Clin Proteomics | Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma Pubmed:35610567 |
| Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB449Bo01 | Recombinant Enolase 1 (ENO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB449Bo01 | Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| LAB449Bo81 | FITC-Linked Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IF. |
| LAB449Bo71 | Biotin-Linked Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC. |
