Cloud-clone
SKU:RPA402Ra04
Recombinant Matrix Metalloproteinase 12 (MMP12)
Recombinant Matrix Metalloproteinase 12 (MMP12)
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Prokaryotic expression
Product No.
RPA402Ra04
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
12.3kDa
Accurate Molecular Mass
14kDa(Analysis of differences refer to the manual)
Residues & Tags
Tyr372~His463 (Accession # NM_053963.2) with N-terminal His Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
9.13
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| International journal of colorectal disease | Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repair Pubmed: 23619615 |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655 |
| American Journal of Physiology - Lung Cellular and Molecular Physiology | Inspiratory resistive breathing induces MMP-9 and MMP-12 expression in the lung Pubmed:25595645 |
| Photodermatol Photoimmunol Photomed. | Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice pubmed:27995657 |
| Яковлева Наталья Юрьевна | |
| Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats | |
| Nat Commun | Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice Pubmed:35031603 |
