Cloud-clone
SKU:RPA603Hu01
Recombinant Apolipoprotein B100 (APOB100)
Recombinant Apolipoprotein B100 (APOB100)
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Prokaryotic expression
Product No.
RPA603Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted, Cytoplasm, Chromosome
Predicted Molecular Mass
21.4kDa
Accurate Molecular Mass
26kDa(Analysis of differences refer to the manual)
Residues & Tags
His3365~Glu3548 with N-terminal His Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
9.5
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| PLoS One | Enhancement of Naringenin Bioavailability by Complexation with Hydroxypropoyl-β-Cyclodextrin Plosone: 0018033 |
| Autonomic Neuroscience | Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic rats ScienceDirect: S1566070211004152 |
| Toxicology | iTRAQ-based proteomic profiling of human serum reveals down-regulation of platelet basic protein and apolipoprotein B100 in patients with hematotoxicity induced by chronic occupational benzene exposure ScienceDirect: S0300483X11004628 |
| Atherosclerosis | Up-reCavia (Guinea pig )lation of Hnf1α gene expression in the liver of rats with experimentally induced chronic renal failure – A possible link between circulating PCSK9 and triacylglycerol concentrations Pubmed:26978583 |
| Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients Pubmed:26680509 |
| molecular nutrition & food research | Alleviating VLDL overproduction is an important mechanism for Laminaria japonica polysaccharide to inhibit atherosclerosis in LDLr-/- mice with diet-induced insulin resistance. pubmed:27928899 |
| PROTEOMICS - Clinical Applications | Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. pubmed:26680509 |
| Biosci Biotechnol Biochem. | Choline and betaine ameliorate liver lipid accumulation induced by vitamin B6 deficiency in rats. pubmed:27696964 |
| Journal of Nutritional Science and Vitaminology | Accumulation of Liver Lipids Induced by Vitamin B6 Deficiency Was Effectively Ameliorated by Choline and, to a Lesser Extent, Betaine Pubmed: 30814419 |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in rats Pubmed: 32105945 |
| Ecotoxicol Environ Saf | Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen … Pubmed: 32911180 |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313 |
| Clinical Nutrition | Postprandial dyslipidemia after a standardized high-fat meal in BMI-matched healthy individuals, and in subjects with prediabetes or Type 2 diabetes 34656950 |
| Nano Research | Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy |
