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Cloud-clone

SKU:RPB831Mu01

Recombinant Hedgehog Homolog, Sonic (SHH)

Recombinant Hedgehog Homolog, Sonic (SHH)

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UOM
Purity
Organism species

Prokaryotic expression

Product No.

RPB831Mu01

Organism Species

Mus musculus (Mouse).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Membrane, Secreted

Predicted Molecular Mass

22.0kDa

Accurate Molecular Mass

20kDa(Analysis of differences refer to the manual)

Residues & Tags

Ser237~His428 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 97%

Isoelectric Point

6.35

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

 

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Annals of the Rheumatic Diseases Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis pubmed:28495672
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB831Mu01 Recombinant Hedgehog Homolog, Sonic (SHH) Positive Control; Immunogen; SDS-PAGE; WB.
APB831Mu01 Active Hedgehog Homolog, Sonic (SHH) Cell culture; Activity Assays.
PAB831Mu01 Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC; IP.
LAB831Mu71 Biotin-Linked Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC.
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