Cloud-clone
SKU:RPB932Hu02
Recombinant Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Recombinant Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
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Prokaryotic expression
Product No.
RPB932Hu02
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Membrane, Nucleus, Cytoplasm
Predicted Molecular Mass
19.5kDa
Accurate Molecular Mass
20kDa(Analysis of differences refer to the manual)
Residues & Tags
Thr154~Val324 with N-terminal His Tag
Buffer Formulation
100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
7.2
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Clin Cancer Res | Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402 |
| Clin Cancer Res | Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402 |
| Diabetes & Metabolism | Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells. pubmed:28499695 |
| Scientific Reports | Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression pubmed:28720868 |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids Pubmed: 30237167 |
| Biomaterials | Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels Pubmed: 30343256 |
| Bulletin of Experimental Biology and Medicine | Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells Pubmed: 30488202 |
| Journal of Medicinal Chemistry | Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral Inhibitors Pubmed: 30938999 |
| International Immunopharmacology | Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway Pubmed: 31078923 |
| Cancers | Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia Pubmed: 31718075 |
| Biological research | Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … Pubmed: 32046779 |
| Biomed Pharmacother | Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway Pubmed: 32028241 |
| Metab Brain Dis | Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats Pubmed: 33170419 |
| South African Journal of Botany | Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo |
| Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice | |
| Cancers (Basel) | BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581 33540760 |
| MOLECULAR CANCER RESEARCH | Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity 33262291 |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026 |
| Cancer Rep (Hoboken) | Curcumin alters distinct molecular pathways in breast cancer subtypes revealed by integrated miRNA/mRNA expression analysis Pubmed:34981672 |
| Prog Neurobiol | Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegeneration Pubmed:35151792 |
| Plant Molecular Biology | Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency Pubmed:35577991 |
