Cloud-clone
SKU:RPD759Ra01
Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC)
Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC)
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Prokaryotic expression
Product No.
RPD759Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Mitochondrion, Cytoplasm
Predicted Molecular Mass
24kDa
Accurate Molecular Mass
25kDa(Analysis of differences refer to the manual)
Residues & Tags
Asp435~Ser636 (Accession # P19468) with N-terminal His Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
5.65
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Oxidative Medicine and Cellular Longevity | Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects pubmed:28798861 |
| Journal of Functional Foods | Effects of maternal serine supplementation on high-fat diet-induced oxidative stress and epigenetic changes in promoters of glutathione synthesis-related genes in … 10.1016:j.jff.2018.05.067 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD759Ra01 | Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD759Ra01 | Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) | WB; IHC; ICC; IP. |
| MAD759Ra21 | Monoclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) | WB; IHC; ICC; IP. |
| SED759Ra | ELISA Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD759Ra | Magnetic Luminex Assay Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
