Cloud-clone
SKU:RPF557Ra02
Recombinant Uncoupling Protein 1, Mitochondrial (UCP1)
Recombinant Uncoupling Protein 1, Mitochondrial (UCP1)
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Prokaryotic expression
Product No.
RPF557Ra02
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Mitochondrion
Predicted Molecular Mass
43kDa
Accurate Molecular Mass
40kDa(Analysis of differences refer to the manual)
Residues & Tags
Pro179~Leu296 (Accession # P04633) with N-terminal His and GST Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
6.35
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Stem Cells | FM19G11 Favors Spinal Cord Injury Regeneration and Stem Cell Self-Renewal by Mitochondrial Uncoupling and Glucose Metabolism Induction PubMed: 22865656 |
| Diabetes. | Inorganic nitrate promotes the browning of white adipose tissue through the nitrate-nitrite-nitric oxide pathway Pubmed:25249574 |
| Journal of Cachexia, Sarcopenia and Muscle | Muscle wasting and adipose tissue browning in infantile nephropathic cystinosis Doi: 10.1002 |
| FASEB J | Single-cell transcriptomics and functional target validation of brown adipocytes show their complex roles in metabolic homeostasis PubMed: 26304220 |
| FASEB JOURNAL | Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and … Pubmed: 32954574 |
| Brown and beige adipose tissue regulate systemic metabolism to resist diet-induced obesity through metabolite signals in an inter-organ signaling axis | |
| Science reports | All-trans-retinoic acid ameliorates atherosclerosis, promotes perivascular adipose tissue browning, and increases adiponectin production in Apo-E mice 33627760 |
| Nature Communications | Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis 33772024 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPF557Ra01 | Recombinant Uncoupling Protein 1, Mitochondrial (UCP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPF557Ra02 | Recombinant Uncoupling Protein 1, Mitochondrial (UCP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF557Ra01 | Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1) | WB; IHC; ICC; IP. |
| PAF557Ra02 | Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1) | WB; IHC; ICC; IP. |
| LAF557Ra71 | Biotin-Linked Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1) | WB; IHC; ICC. |
| SEF557Ra | ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF557Ra | Magnetic Luminex Assay Kit for Uncoupling Protein 1, Mitochondrial (UCP1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
