Cloud-clone
SKU:RPB342Mi01
Recombinant Actin Alpha 2, Smooth Muscle (ACTa2)
Recombinant Actin Alpha 2, Smooth Muscle (ACTa2)
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Prokaryotic expression
Product No.
RPB342Mi01
Organism Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Cytoplasm
Predicted Molecular Mass
27.6kDa
Accurate Molecular Mass
27kDa(Analysis of differences refer to the manual)
Residues & Tags
Ile167~Phe377 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
6.2
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Cell Mol Immunol | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496 |
| NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY | Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats Pubmed: 32936352 |
| Brazilian Journal of Pharmaceutical Sciences | Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm |
| Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells | |
| Parasit Vectors | Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate … 34857049 |
| Clinical and Translational Medicine | NOX5 mediates the crosstalk between tumor cells and cancer‐associated fibroblasts via regulating cytokine network 34459125 |
