Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1)

Prokaryotic expression

Product No.

RPB358Mu01

Organism Species

Mus musculus (Mouse).

Source                                                                      

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Cytoplasm

Predicted Molecular Mass

30.7kDa

Accurate Molecular Mass

31kDa(Analysis of differences refer to the manual)

Residues & Tags

Leu693~Arg933 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 95%

Isoelectric Point

8.6

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine	Citations
Fish Physiology and Biochemistry	Heat-shock protein 70 modulates apoptosis signal-regulating kinase 1 in stressed hepatocytes of Mugil cephalus Pubmed:24875452
Fish Physiology and Biochemistry	Differential reCavia (Guinea pig )lation of pro- and antiapoptotic proteins in fish adipocytes during hypoxic conditions. Pubmed:26744268
网页打不开	ASK-1 Expression Down-regulated in Preeclamptic Placental Explants Incubated with Mint-Tea :
Mar Pollut Bull.	Differential expression of survival proteins during decreased intracellular oxygen tension in brain endothelial cells of grey mullets. pubmed:28043644
Journal of FisheriesSciences. com	Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stress b0407ebde042c4f61b728bc11a586e6c