Cloud-clone
SKU:RPA289Mi01
Recombinant Histone H4 (H4)
Recombinant Histone H4 (H4)
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Prokaryotic expression
Product No.
RPA289Mi01
Organism Species
Mus musculus (Mouse).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method).
Subcellular Location
Secreted
Predicted Molecular Mass
41.23kDa
Accurate Molecular Mass
44kDa(Analysis of differences refer to the manual)
Residues & Tags
Ser2~Gly103 with N-terminal His and GST Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 80%
Isoelectric Point
12.8
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| PLoS One. | N-Acetyl-Heparin Attenuates Acute Lung Injury Caused by Acid Aspiration Mainly by Antagonizing Histones in Mice Pubmed:24816808 |
| Crit Care. | Impact of plasma histones in human sepsis and their contribution to cellular injury and inflammation Pubmed:25260379 |
| Biomed Res Int | A Novel Anti-Histone H2 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649891 |
| Journal of Viral Hepatitis | Patients with HBV-related acute-on-chronic liver failure have increased concentrations of extracellular histones aggravating cellular damage and systemic inflammation pubmed:27660136 |
| Cell Death & Disease | Dexmedetomidine inhibits astrocyte pyroptosis and subsequently protects the brain in in vitro and in vivo models of sepsis Pubmed: 30778043 |
| Respir Res | Histone H4 induces heparan sulfate degradation by activating heparanase in chlorine gas-induced acute respiratory distress syndrome Pubmed:35073921 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA289Mi02 | Recombinant Histone H4 (H4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA289Mi01 | Recombinant Histone H4 (H4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA289Mi02 | Polyclonal Antibody to Histone H4 (H4) | WB; IHC; ICC; IP. |
| PAA289Mi01 | Polyclonal Antibody to Histone H4 (H4) | WB; IHC; ICC; IP. |
| SEA289Mi | ELISA Kit for Histone H4 (H4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA289Mi | High Sensitive ELISA Kit for Histone H4 (H4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA289Mi | Magnetic Luminex Assay Kit for Histone H4 (H4) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
