Cloud-clone
SKU:RPE132Hu01
Recombinant Defensin Beta 103A (DEFb103A)
Recombinant Defensin Beta 103A (DEFb103A)
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Prokaryotic expression
Product No.
RPE132Hu01
Organism Species
Homo sapiens (Human).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
8.9kDa
Accurate Molecular Mass
12kDa(Analysis of differences refer to the manual)
Residues & Tags
Gly23~Lys67 with N-terminal His Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
10.3
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Plos Pathogens | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in?Pseudomonas aeruginosa?Keratitis PubMed: PMC3715414 |
| Scientific Reports | Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique Pubmed:29374283 |
| PeerJ | Antibacterial activity of human defensins against Staphylococcus aureus and Escherichia coli 33304659 |
| Animals | Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport 33375218 |
| Sci Rep | Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus 34362937 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APE132Hu01 | Active Defensin Beta 103A (DEFb103A) | Cell culture; Activity Assays. |
| RPE132Hu01 | Recombinant Defensin Beta 103A (DEFb103A) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE132Hu01 | Polyclonal Antibody to Defensin Beta 103A (DEFb103A) | WB |
| MAE132Hu21 | Monoclonal Antibody to Defensin Beta 103A (DEFb103A) | WB; IHC; ICC; IP. |
| SEE132Hu | ELISA Kit for Defensin Beta 103A (DEFb103A) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME132Hu | Magnetic Luminex Assay Kit for Defensin Beta 103A (DEFb103A) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
