Cloud-clone
SKU:RPB286Ra01
Recombinant Milk Fat Globule EGF Factor 8 (MFGE8)
Recombinant Milk Fat Globule EGF Factor 8 (MFGE8)
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Prokaryotic expression
Product No.
RPB286Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Membrane, Secreted
Predicted Molecular Mass
69.4kDa
Accurate Molecular Mass
69kDa(Analysis of differences refer to the manual)
Residues & Tags
Cys73~Arg421 with N-terminal His and GST Tag
Buffer Formulation
100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 90%
Isoelectric Point
8.6
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Fertility and Sterility | Tumor necrosis factor α up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor κB activation, resulting in cell migration of epithelial cells. Pubmed: 24262600 |
| U.S. Patent Application | Biomarker for amyloid-b-related neurological disorders US20130302838 |
| IUBMB Life. | Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status. pubmed:28067023 |
| Journal of diabetes investigation | Circulating milk fat globule-epidermal growth factor 8 levels are increased in pregnancy and gestational diabetes mellitus. pubmed:28035763 |
| The Journal of Steroid Biochemistry and Molecular Biology | Modifications of Western-type diet regarding protein, fat and sucrose levels as modulators of steroid metabolism and activity in liver pubmed:27471150 |
| Journal of Steroid Biochemistry and Molecular Biology | Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910 |
| Oncotarget | Pantoprazole blocks the JAK2/STAT3 pathway to alleviate skeletal muscle wasting in cancer cachexia by inhibiting inflammatory response. pubmed:28489606 |
| Molecular and Cellular Neuroscience | TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway 10.1016/j.mcn.2017.08.006 |
| Amyloid | Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein Pubmed: 31615282 |
| clinical and translational medicine | Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis 33634976 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB286Ra01 | Recombinant Milk Fat Globule EGF Factor 8 (MFGE8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB286Ra01 | Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) | WB; IHC; ICC; IP. |
| MAB286Ra21 | Monoclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) | WB; IHC; ICC; IP. |
| SEB286Ra | ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB286Ra | Magnetic Luminex Assay Kit for Milk Fat Globule EGF Factor 8 (MFGE8) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
