Cloud-clone
SKU:RPC243Mu01
Recombinant Topoisomerase I (TOP1)
Recombinant Topoisomerase I (TOP1)
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Prokaryotic expression
Product No.
RPC243Mu01
Organism Species
Mus musculus (Mouse).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Nucleus
Predicted Molecular Mass
34.1kDa
Accurate Molecular Mass
34kDa(Analysis of differences refer to the manual)
Residues & Tags
Met1~Ser252 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
10.1
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Electronic Journal of Biotechnology | Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris science:S0717345816000233 |
| Journal of Experimental & Clinical Cancer Research | Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II pubmed:27852319 |
| Tikrit Journal of Pure Science | Partial purification of topoisomerase IB from serum of diabetic patients and study it's kinetic properties and molecular weight |
| EurAsian Journal of BioSciences | Study of topoisomerase I (Topo I) level and some biochemical parameters in diabetic patients |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC243Mu01 | Recombinant Topoisomerase I (TOP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC243Mu02 | Recombinant Topoisomerase I (TOP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC243Mu01 | Polyclonal Antibody to Topoisomerase I (TOP1) | WB; IHC; ICC; IP. |
| PAC243Mu02 | Polyclonal Antibody to Topoisomerase I (TOP1) | WB; IHC; ICC; IP. |
| SEC243Mu | ELISA Kit for Topoisomerase I (TOP1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC243Mu | Magnetic Luminex Assay Kit for Topoisomerase I (TOP1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
