Cloud-clone
SKU:RPC284Ra01
Recombinant Afamin (AFM)
Recombinant Afamin (AFM)
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Prokaryotic expression
Product No.
RPC284Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
24.4kDa
Accurate Molecular Mass
15&26kDa(Analysis of differences refer to the manual)
Residues & Tags
Leu22~Tyr201 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
5.7
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Journal of Proteomics | 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer ScienceDirect: S1874391912004964 |
| Clin Chim Acta | Afamin — A pleiotropic glycoprotein involved in various disease states PubMed: 25892677 |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … Pubmed:30037068 |
| Biomarkers in Medicine | Urine afamin and afamin–creatinine ratio as biomarkers for kidney injury Pubmed: 30433819 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC284Ra01 | Recombinant Afamin (AFM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPC284Ra21 | OVA Conjugated Afamin (AFM) | Immunogen; SDS-PAGE; WB. |
| PAC284Ra01 | Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
| PAC284Ra08 | Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
| MAC284Ra21 | Monoclonal Antibody to Afamin (AFM) | WB |
| MAC284Ra24 | Monoclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
| MAC284Ra25 | Monoclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
| MAC284Ra22 | Monoclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
| MAC284Ra23 | Monoclonal Antibody to Afamin (AFM) | WB |
| CEC284Ra | ELISA Kit for Afamin (AFM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC284Ra | Magnetic Luminex Assay Kit for Afamin (AFM) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
