Cloud-clone
SKU:RPC128Ra01
Recombinant Gremlin 1 (GREM1)
Recombinant Gremlin 1 (GREM1)
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Prokaryotic expression
Product No.
RPC128Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
22.0kDa
Accurate Molecular Mass
22kDa(Analysis of differences refer to the manual)
Residues & Tags
Lys25~Asp184 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
9.6
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Lung | Intrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial Hypertension PubMed: 25926293 |
| Tumour Biol | Overexpression of Gremlin promotes non-small cell lung cancer progression PubMed: 26392110 |
| Int J Cardiol | Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patients PubMed: 26539964 |
| International Journal of Cardiology | Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-termprognosis in suspected and proven acute heart failure patients Pubmed:26539964 |
| American Journal of Respiratory Cell and Molecular Biology | BMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAH Pubmed:27187737 |
| J Rheumatol | GREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and Invasiveness Pubmed:26834210 |
| European cells & materials | The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. pubmed:28266688 |
| Sci Rep. | Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients. pubmed:28327551 |
| American Journal of Respiratory Cell and Molecular Biology | Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial … rcmb.2015-0402OC |
| Molecular Medicine Reports | Gremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cells pubmed:28260028 |
| Cellular Haemostasis and Platelets | Platelets as a novel source of Gremlin-1: Implications for thrombo inflammation pubmed:27929199 |
| Scientific Reports | Gremlin, A Potential Urinary Biomarker of Anca-Associated Crescentic Glomerulonephritis Pubmed: 31053735 |
| International Journal of Molecular Sciences | Transcriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage Regeneration Pubmed: 31878307 |
| American Journal of Physiology-Cell Physiology | Suppression of microRNA-23a/b-3p delays the development degenerative joint disease by activating Grem1 and TGF-β/Smad signaling pathway Pubmed: 32186934 |
| Inflammopharmacology | Protective role of microRNA-23a/b-3p inhibition against osteoarthritis through Gremlin1-depenent activation of TGF-β/smad signaling in chondrocytesa Pubmed:35441352 |
| Mol Carcinog | CAF‐derived exosomes transmitted Gremlin‐1 promotes cancer progression and decreases the sensitivity of hepatoma cells to sorafenib Pubmed:35638711 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| APC128Ra01 | Active Gremlin 1 (GREM1) | Cell culture; Activity Assays. |
| RPC128Ra01 | Recombinant Gremlin 1 (GREM1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC128Ra01 | Polyclonal Antibody to Gremlin 1 (GREM1) | WB; IHC; ICC; IP. |
| SEC128Ra | ELISA Kit for Gremlin 1 (GREM1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC128Ra | Magnetic Luminex Assay Kit for Gremlin 1 (GREM1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| PGC128Ra01 | Primer Pair for Gremlin 1 (GREM1) | PCR |
