Cloud-clone
SKU:RPA710Ra01
Recombinant Prothrombin Fragment 1+2 (F1+2)
Recombinant Prothrombin Fragment 1+2 (F1+2)
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Prokaryotic expression
Product No.
RPA710Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Extracellular matrix
Predicted Molecular Mass
35.4kDa
Accurate Molecular Mass
40kDa(Analysis of differences refer to the manual)
Residues & Tags
Ala44~Arg323 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
5.1
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Critical Care | Disseminated intravascular coagulation or acute coagulopathy of trauma shock early after trauma? An observational study BioMed: cc10553 |
| Journal of Vascular Surgery | Changes in thrombin generation, fibrinolysis, platelet and endothelial cell activity, and inflammation following endovascular abdominal aortic aneurysm repair ScienceDirect: S0741521411018568 |
| Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine | High levels of soluble VEGF receptor 1 early after trauma are associated with shock, sympathoadrenal activation, glycocalyx degradation and inflammation in severely injured patients: a prospective study Sjtrem:1757-7241 |
| Journal of Thrombosis and Haemostasis | High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality Wiley: source |
| British Journal of Dermatology | Evaluation of autologous plasma skin test in patients with chronic idiopathic urticaria PubMed: 21910697 |
| Arteriosclerosis, Thrombosis, and Vascular Biology. | Diet Modulates Endogenous Thrombin Generation, A Biological Estimate of Thrombosis Risk, Independently of the Metabolic Status PubMed: 22859493 |
| Journal of Vascular Surgery | Medium-term effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis PubMed: 23140799 |
| PLoS ONE | Markers of Thrombogenesis and Fibrinolysis and Their Relation to Inflammation and Endothelial Activation in Patients with Idiopathic Pulmonary Arterial Hypertension Plosone: Source |
| Journal of Vascular Surgery | Effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis Pubmed: 23140799 |
| Haemetology | Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India Pubmed: 23992124 |
| Cogent Medicine | Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunction Tandfonline:Source |
| Interact Cardiovasc Thorac Surg. | An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dose Pubmed:24632424 |
| Perfusion | Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing PubMed: 26243277 |
| J Thromb Haemost | Comparison of the Effect of Dabigatran and Dalteparin on Thrombus Stability in a Murine Model of Venous Thromboembolism PubMed: 26514101 |
| Acta Oncologica Turcica | Thrombin activatable fibrinolysis inhibitor (TAFI), tissue factor pathway inhibitor (TFPI), and prothrombin fragment 1+2 levels in patients with advanced colorectal cancer aot:AOT_49_1_6_12.pdf |
| Advances in Medical Sciences | Perioperative thrombocytopenia predicts poor outcome in patients undergoing transcatheter aortic valve implantation pubmed:29145170 |
| Thrombosis Research | The causes of thrombocytopenia after transcatheter aortic valve implantation pubmed:28582640 |
| Acta Haematologica | Elevations of Thrombotic Biomarkers in Hemoglobin H Disease Pubmed:29402840 |
| Journal of Neurotrauma | The cerebral thrombin system is activated after intracerebral hemorrhage and contributes to secondary lesion growth and poor neurological outcome in C57Bl/6 mice Pubmed: 31830857 |
| Indian Journal of Public Health Research & Development | Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever |
| Rheumatol Int | The impact of anti-endothelial cell antibodies (AECAs) on the development of blood vessel damage in patients with systemic lupus erythematosus: the preliminary … Pubmed:35284968 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA710Ra01 | Recombinant Prothrombin Fragment 1+2 (F1+2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA710Ra01 | Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2) | WB; IHC; ICC; IP. |
| LAA710Ra81 | FITC-Linked Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2) | WB; IHC; ICC; IF. |
| LAA710Ra71 | Biotin-Linked Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2) | WB; IHC; ICC. |
| SEA710Ra | ELISA Kit for Prothrombin Fragment 1+2 (F1+2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA710Ra | CLIA Kit for Prothrombin Fragment 1+2 (F1+2) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA710Ra | Magnetic Luminex Assay Kit for Prothrombin Fragment 1+2 (F1+2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA710Ra01 | ELISA Kit DIY Materials for Prothrombin Fragment 1+2 (F1+2) | Main materials for "Do It (ELISA Kit) Yourself". |
